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Determination of platelet activation markers expression in the pathophysiology of thrombotic states
Řádek, Martin ; Kvasnička, Tomáš (advisor) ; Dulíček, Petr (referee) ; Říhová, Lucie (referee)
Background/Aims: Thrombophilia, ie an increased predisposition to venous and arterial thrombosis, is a complex disease caused by disorders of platelets and endothelial, among others. Circulating endothelial cells (CEC) and endothelial progenitor cells (EPC) have been described as markers of endothelial damage and dysfunction, respectively as markers of its recovery in many diseases, including thrombotic complications. However, their significance in patients with known thrombophilia has not yet been investigated. Both CEC and EPC represent extremely rare cell populations found in peripheral blood. Therefore, it is essential to use exclusively standardized and sensitive methods for their identification and quantification. The aim of the study was to identify and quantify CEC and EPC in the peripheral blood of patients with congenital thrombophilia and to evaluate their importance as markers of endothelial and platelet activity in context with the risk of thrombosis occuring and recurrence. Methods: Analysis of the number of CEC and EPC in the peripheral blood of patients with thrombophilia with or without a history of thrombosis and patients with acute thrombosis was performed by multicolor flow cytometry. The CEC and EPC reference values were determined on a group of healthy controls. Patients with...
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